Adikay Sreedevi; Sravana Sangeetha; Kamsali Murali Mohan Achari; Kaveripakam Sai Sruthi; Yellamandayya Vadlamudi
Volume 4, Issue 8 , August 2022, , Pages 768-777
Abstract
Exploring the phytochemicals from traditional medicinal plants is essential for developing the novel leads for various diseases. In humans, many diseases are associated with the accumulation ...
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Exploring the phytochemicals from traditional medicinal plants is essential for developing the novel leads for various diseases. In humans, many diseases are associated with the accumulation of free radicals. Antioxidants have the competence to scavenge free radicals and keep down their impact. Hence in the current study, we intended the identification of phytoconstituents by aiding HPLC and GC-MS methods and assessing the antioxidant ability of ethanol extract of roots of Jasminum auriculatum using in vitro and in silico approaches. Ethanol extract of roots of Jasminum auriculatum was prepared and inflicted to the preliminary phytochemical studies followed by HPLC and GC -MS analysis. Further in vitro and in silico studies were conducted to assess antioxidant action of ethanol extract. The results of HPLC analysis led to the presence of rutin and GC-MS analysis resulted in presence of various bioactive compounds. In vitro evaluation of free radical scavenging ability using DPPH, NO and ABTS techniques exhibited that the root extract possess good antioxidant activity. Further molecular docking studies performed for the identified compounds in HPLC and GC MS analysis, which substantiated the in vitro studies by resulting in good glide docking score with the protein targets namely NADPH oxidase and Super Oxide Dismutase. Based on these experimental findings, it can be concluded that the roots of Jasminum auriculatum possess good antioxidant activity.